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Goat Anti-C16orf57 (aa154-167) Antibody (EB11248)

Related Products: EBP11248 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11248 Goat Anti-C16orf57 (aa154-167) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11248 1
EB11248 (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11248 2
EB11248 (0.1µg/ml) staining of Mouse (A) and Rat (B) Skin lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: chromosome 16 open reading frame 57, FLJ13154, HVSL motif containing 1, HVSL1, hypothetical protein LOC79650, PN, C16orf57
Official Symbol: USB1
Accession Number(s): NP_078874.2; NP_001182231.1
Human GeneID(s): 79650
Important Comments: This antibody is expected to recognize both reported isoforms (NP_078874.2; NP_001182231.1).

Immunogen

Peptide with sequence C-TANQVKIYTNQEKT, from the internal region of the protein sequence according to NP_078874.2; NP_001182231.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35+28kDa bands observed in Human, Mouse and Rat Skin lysates (calculated MW of 30.3kDa according to NP_078874.2 and 28.1kDa according to NP_001182231.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig

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