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Goat Anti-Calnexin Antibody (EB09525)

Code Name Applications Availability Size Tested species Price Grade  
EB09525 Goat Anti-Calnexin Antibody Pep-ELISA, WB, IHC, IF Allow up to 10 days for delivery 100µg specific antibody in 200µl Human, Mouse $333.00 Elite

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ARP American Research Products, Inc
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EB09525 1
EB09525 (0.1µg/ml) staining of Human Cerebellum (A) and Colorectal cancer (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09525 2
EB09525 (0.1µg/ml) staining of CaCo-2 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09525 3
EB09525 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09525 4
EB09525 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88
Official Symbol: CANX
Accession Number(s): NP_001737.1
Human GeneID(s): 821
Non-Human GeneID(s): 12330 (mouse), 29144 (rat)
Important Comments: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Immunogen

Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 90kDa band observed in Human Colorectal cancer lysates and lysates of cell line NIH3T3, and approx 100kDa in Human Cerebellum lysates and in lysates of cell line CaCo-2 (calculated MW of 67.6kDa according to Human NP_001737.1 and ). These molecular weights are routinely observed by other sources, Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum (ER) of NIH3T3 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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