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Code Name Applications Availability Size Tested species Price Grade  
EB12421 Goat Anti-CAPG Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12421 1
EB12421 (0.03µg/ml) staining of THP-1 (A) and (0.3µg/ml) U937 (B) and A431 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12421 2
EB12421 (0.1µg/ml) staining of Human Spleen (A) and Tonsil (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12421 3
EB12421 Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAPG, capping protein (actin filament), gelsolin-like, AFCP, MCP, actin regulatory protein CAP-G, actin-regulatory protein CAP-G, gelsolin-like capping protein, macrophage capping protein, macrophage-capping protein
Official Symbol: CAPG
Accession Number(s): NP_001738.2; NP_001243069.1; NP_001307663.1
Human GeneID(s): 822
Important Comments: This antibody is expected to recognize all reported isoform (NP_001738.2; NP_001243069.1, NP_001307663.1. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.

Immunogen

Peptide with sequence C-NIRATERALNWDS, from the internal region of the protein sequence according to NP_001738.2; NP_001243069.1; NP_001307663.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 38kDa band observed in lysates of cell lines THP-1, U937 and A431, and in Human Spleen lysates and approx. 37kDa in Human Tonsil lysates (calculated MW of 38.5kDa according to NP_001738.2 and 36.9kDa according to NP_001243069.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.


Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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