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Goat Anti-CAPN1 (aa468-481) Antibody (EB11989)

Related Products: EBP11988 - Immunizing Peptide, EBP11989 - Immunizing Peptide, Goat Anti-CAPN1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11989 Goat Anti-CAPN1 (aa468-481) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11989 1
EB11989 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11989 2
EB11989 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAPN1, calpain 1, (mu/I) large subunit, CANP, CANP1, CANPL1, muCANP, muCL, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 catalytic subunit, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30, micromolar-calpain
Official Symbol: CAPN1
Accession Number(s): NP_005177.2
Human GeneID(s): 823
Important Comments: Reported variants represent identical protein: NP_001185797.1, NP_005177.2, NP_001185798.1.

Immunogen

Peptide with sequence C-NASRARSEQFINLR, from the internal region of the protein sequence according to NP_005177.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in Human Kidney lysates (calculated MW of 81.9kDa according to NP_005177.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow

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