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Goat Anti-cardiac troponin T (aa201-213) Antibody (EB11809)

Related Products: EBP11809 - Immunizing Peptide, EBP11810 - Immunizing Peptide, Goat Anti-cardiac troponin T Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11809 Goat Anti-cardiac troponin T (aa201-213) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11809 1
EB11809 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11809 2
EB11809 (0.1µg/ml) staining of Pig Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: cardiac muscle troponin T, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, CMH2, CMPD2, cTnT, LVNC6, MGC3889, OTTHUMP00000033864, OTTHUMP00000033865, OTTHUMP00000033866, OTTHUMP00000033867, OTTHUMP00000033870, OTTHUMP00000218095, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), troponin T, cardiac muscle, troponin T2, cardiac
Official Symbol: TNNT2
Accession Number(s): NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1
Human GeneID(s): 7139
Non-Human GeneID(s): 21956 (mouse), 24837 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1).

Immunogen

Peptide with sequence C-TERKSGKRQTERE, from the internal region of the protein sequence according to NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in Human, Mouse and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID: 20083571). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Pig
Expected from sequence similarity: Human, Mouse, Rat, Cat, Dog, Pig, Cow

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