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Goat Anti-CAV3 Antibody (EB09304)

Related Products: EBP09304 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09304 Goat Anti-CAV3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09304 1
B09304 (0.1µg/ml) staining of Human Skeletal Muscle (A) and Rat Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09304 2
EB09304 (4µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09304 3
EB09304 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAV3, caveolin 3, LGMD1C, LQT9, MGC126100, MGC126101, MGC126129, VIP-21, VIP21, M-caveolin
Official Symbol: CAV3
Accession Number(s): NP_203123.1
Human GeneID(s): 859
Non-Human GeneID(s): 12391 (mouse), 29161 (rat)
Important Comments: Reported variants represent identical protein (NP_001225.1; NP_203123.1).

Immunogen

Peptide with sequence EHTDLEAQIVKDIH-C, from the N Terminus of the protein sequence according to NP_203123.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 23kDa band observed in Human Skeletal Muscle and Rat Heart lysates and in preliminary testing of Mouse Skeletal Muscle lysate (calculated MW of 17.3kDa according to Human NP_203123.1, and 17.4kDa according to , Rat NP_062028.1 . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Heart. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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