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Code Name Applications Availability Size Tested species Price Grade  
EB10961-T Goat Anti-CB1 (isoform a) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB10961-T 1
EB10961 (0.3µg/ml) staining of A549 (A) and NIH3T3 (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CNR1, cannabinoid receptor 1 (brain), CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR, cannabinoid receptor 1, central cannabinoid receptor
Official Symbol: CNR1
Accession Number(s): NP_057167.2
Human GeneID(s): 1268
Non-Human GeneID(s): 12801 (mouse), 25248 (rat)
Important Comments: This antibody is expected to recognize reported isoform a (NP_057167.2) only. Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1

Immunogen

Peptide with sequence SNDIQYEDIKGDMAS-C, from the N Terminus of the protein sequence according to NP_057167.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 52kDa band observed in lysates of cell lines A549 and NIH3T3 (calculated MW of 52.9kDa according to Human NP_057167.2 and 52.8kDa according to Mouse NP_031752.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: 33049367 and http://doi.org/10.1089/can.2020.0107.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

This antibody has been successfully used in WB on Human:
Melissa L. Kozakiewicz, Jie Zhang, Sandra Leone-Kabler, Liliya M. Yamaleyeva, Anna G. McDonald, Brian C. Brost and Allyn C. Howlett
Differential Expression of CB1 Cannabinoid Receptor and Cannabinoid Receptor Interacting Protein 1a in Labor
Cannabis and Cannabinoid Research (April 2021) http://doi.org/10.1089/can.2020.0107
PMID: 33998898

This antibody has been successfully used in WB on Human:
Erica L Lyons, Sandra Leone-Kabler, Alexander L Kovach, Brian F Thomas, Allyn C Howlett
Cannabinoid receptor subtype influence on neuritogenesis in human SH-SY5Y cells
Mol Cell Neurosci. 2020 Oct 10;109:103566.
PMID: 33049367

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