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Code Name Applications Availability Size Tested species Price Grade  
EB06959-T Goat Anti-CBX3 / HP1 Gamma Antibody - Trial Pep-ELISA, WB, IHC, IF, FC Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06959-T 1
EB06959 (0.03µg/ml) staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06959-T 2
EB06959 (0.1µg/ml) staining of NIH3T3 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06959-T 3
EB06959 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06959-T 4
EB06959 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06959-T 5
EB06959 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06959-T 6
EB06959 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila), HGNC:1553, HECH, HP1Hs-gamma, HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1
Official Symbol: CBX3
Accession Number(s): NP_009207.2
Human GeneID(s): 11335
Non-Human GeneID(s): 12417 (mouse), 297093 (rat)
Important Comments: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Immunogen

Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 µg/ml.
IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3µg/ml.

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Francescopaolo Di Cello, James Shin, Kirsten Harbom, Cory Brayton
Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice
Biochem Biophys Res Commun. 2013 Apr 26;434(1):70-4.
PMID: 23545254

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