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Code Name Applications Availability Size Tested species Price Grade  
EB06959-B Goat Anti-CBX3 / HP1 Gamma, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06959-B 1
Biotinylated EB06959 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: CBX3, chromobox 3, HECH, HP1-GAMMA, HP1Hs-gamma, HP1 gamma homolog, chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, modifier 2 protein
Official Symbol: CBX3
Accession Number(s): NP_009207.2
Human GeneID(s): 11335
Non-Human GeneID(s): 12417 (mouse), 297093 (rat)
Important Comments: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Immunogen

Peptide with sequence CEAFLNSQKAGKEKD., from the internal region of the protein sequence according to NP_009207.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 20kDa band observed in lysates of cell line NIH3T3 (calculated MW of 20.8kDa according to Mouse NP_031650.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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