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Goat Anti-CBX5 / HP1-Alpha, biotinylated Antibody (EB06957-B)

Related Products: EBP06957-B - Immunizing Peptide, EBP06957 - Immunizing Peptide, Goat Anti-CBX5 / HP1-Alpha Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06957-B Goat Anti-CBX5 / HP1-Alpha, biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06957-B 1
Biotinylated EB06957 (0.1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: CBX5, chromobox homolog 5, HEL25, HP1, HP1A, HP1 alpha homolog, HP1-ALPHA, HP1Hs alpha, antigen p25, chromobox homolog 5 (HP1 alpha homolog, Drosophila), epididymis luminal protein 25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha
Official Symbol: CBX5
Accession Number(s): NP_036249.1
Human GeneID(s): 23468
Important Comments: Reported variants represent identical protein: NP_036249.1, NP_001120793.1, NP_001120794.1

Immunogen

Peptide with sequence C-NKRKSNFSNSADDIK., from the internal region of the protein sequence according to NP_036249.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 25kDa band observed in HeLa lysates (calculated MW of 22.2kDa according to NP_036249.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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