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Goat Anti-CD3-ZETA / CD247 Antibody (EB12595)

Related Products: EBP12595 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12595 Goat Anti-CD3-ZETA / CD247 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12595 1
EB12595 (0.1µg/ml) staining of Jurkat (A) and (0.3ug/ml) MOLT4 (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD247, CD247 molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain
Official Symbol: CD247
Accession Number(s): NP_932170.1; NP_000725.1
Human GeneID(s): 919
Important Comments: This antibody is expected to recognize both reported isoforms (NP_932170.1; NP_000725.1).

Immunogen

Peptide with sequence C-KNPQEGLYNELQKD, from the internal region of the protein sequence according to NP_932170.1; NP_000725.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 16-17kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6kDa according to NP_000725.1). Recommended concentration: 0.1-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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