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Goat Anti-CD86 / B7-2 (aa 73-87) Antibody (EB10739)

Code Name Applications Availability Size Tested species Price Grade  
EB10739 Goat Anti-CD86 / B7-2 (aa 73-87) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB10739 1
EB10739 (6µg/ml) staining of paraffin embedded Human Lymph Node. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB10739 2
EB10739 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
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Target Protein

Principal Names: CD86, CD86 molecule, B7-2, B70, CD28LG2, LAB72, MGC34413, B-lymphocyte activation antigen B7-2, B7-2 antigen, CD86 antigen, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CTLA-4 counter-receptor B7.2
Official Symbol: CD86
Accession Number(s): NP_787058.5; NP_008820.4; NP_795711.2
Human GeneID(s): 942
Important Comments: This antibody is expected to recognize reported isoforms (NP_787058.5 (isoform 1), NP_008820.4 (isoform 2) and NP_795711.2 (isoform 3).

Immunogen

Peptide with sequence C-EKFDSVHSKYMGRTS, from the internal region of the protein sequence according to NP_787058.5; NP_008820.4; NP_795711.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
IHC: Paraffin embedded Human Lymph Node. Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human