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Goat Anti-Cd97 (mouse) Antibody (EB11876)

Related Products: EBP11876 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11876 Goat Anti-Cd97 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
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Email: [email protected]
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EB11876 1
EB11876 (0.3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11876 2
EB11876 (1µg/ml) staining of Mouse Colon (wt, left lane, knock-out right lane) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by infrared fluorescence (Odyssey). Data obtained from anonymous customer
Click here to download PDF datasheet.

Target Protein

Principal Names: Cd97, CD97 antigen, AA409984, TM7LN1, EGF-TM7 receptor, adhesion G protein-coupled receptor E5
Official Symbol: Adgre5
Accession Number(s): NP_036055.2; NP_001156501.1; NP_001156502.1; NP_001156503.1
Non-Human GeneID(s): 26364 (mouse)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_036055.2; NP_001156501.1; NP_001156502.1; NP_001156503.1).

Immunogen

Peptide with sequence C-TSQTRALRSSES, from the C Terminus of the protein sequence according to NP_036055.2; NP_001156501.1; NP_001156502.1; NP_001156503.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in Mouse Thymus and Spleen lysates (calculated MW of 80.2kDa according to NP_001156503.1). The beta chain is detected as a ~30kDa band in Mouse Colon (transgenic overexpressing Cd76 in intestines) and not in the knock-out. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse

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