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Goat Anti-CDC48 / YDL126C Antibody (EB11800)

Related Products: EBP11800 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11800 Goat Anti-CDC48 / YDL126C Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB11800 1
EB11800 (0.01µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11800 2
EB11800 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDC48, CDC48p, transitional endoplasmic reticulum ATPase, VCP, YDL126C, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA
Official Symbol: CDC48
Accession Number(s): NP_010157.1
Human GeneID(s): 7415
Non-Human GeneID(s): 269523 (mouse), 116643 (rat)
Important Comments: This antibody may cross react in many other species.

Immunogen

Peptide with sequence C-HTKNMKLADDVDLE, from the internal region of the protein sequence according to NP_010157.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Saccharomyces cerevisiae S288c; Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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