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Goat Anti-CDKAL1 Antibody (EB08288)

Related Products: EBP08288 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08288 Goat Anti-CDKAL1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: CDK5 regulatory subunit associated protein 1-like 1, FLJ20342, FLJ46705, MGC75469
Official Symbol: CDKAL1
Accession Number(s): NP_060244.2
Human GeneID(s): 54901
Non-Human GeneID(s): 68916 (mouse), 361243 (rat)

Immunogen

Peptide with sequence C-QVKKQRTKDLSR, from the internal region of the protein sequence according to NP_060244.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells . Preliminary experiments gave an approx. 39kDa band in Human Brain (Cerebellum) lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

Brambillasca S, Altkrueger A, Colombo SF, Friederich A, Eickelmann P, Mark M, Borgese N, Solimena M.
CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.
J Biol Chem. 2012 Dec 7;287(50):41808-19.
PMID: 23048041

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