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Goat Anti-CEP290 / NPHP6 Antibody (EB08384)

Code Name Applications Availability Size Tested species Price Grade  
EB08384 Goat Anti-CEP290 / NPHP6 Antibody Pep-ELISA, IHC, IF Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB08384 1
EB08384 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB08384 2
EB08384 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
EB08384 3
EB08384 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and centrosome staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: CEP290, centrosomal protein 290kDa, 3H11Ag, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16, CTCL tumor antigen se2-2, monoclonal antibody 3H11 antigen, nephrocystin 6, nephrocystin-6, nephrocytsin-6, prostate cancer antigen T21
Official Symbol: CEP290
Accession Number(s): NP_079390.3
Human GeneID(s): 80184

Immunogen

Peptide with sequence C-QSGAESTIPDADQ, from the internal region of the protein sequence according to NP_079390.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 4-8µg/ml.

Immunofluorescence: Strong expression of the protein seen within the centrosome of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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