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Goat Anti-CES1 Antibody (EB08405)

Related Products: EBP08405 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08405 Goat Anti-CES1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08405 1
EB08405 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08405 2
EB08405 (2.5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn, liver carboxylesterase, triacylglycerol hydrolase, monocyte/macrophage serine-esterase 1
Official Symbol: CES1
Accession Number(s): NP_001020366.1; NP_001020365.1; NP_001257.4
Human GeneID(s): 1066
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).

Immunogen

Peptide with sequence C-NTQAAQKLKDKE, from the internal region (near C terminus) of the protein sequence according to NP_001020366.1; NP_001020365.1; NP_001257.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.
IHC: In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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