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Goat Anti-CES1 Antibody (EB08405)

Related Products: EBP08405 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08405 Goat Anti-CES1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08405 1

EB08405 optimised QC. Primary incubation 1 hour at room temperature.

Image A: THP-1 cell lysate at primary Ab concentration 1µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB08405 2

EB08405 optimised QC. Primary incubation 1 hour at room temperature.

Image A: Human Duodenum lysate at primary Ab concentration 1µg/ml. Image B: Human Liver lysate at primary Ab concentration 0.5ug/ml (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB08405 3
EB08405 (2.5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn, liver carboxylesterase, triacylglycerol hydrolase, monocyte/macrophage serine-esterase 1
Official Symbol: CES1
Accession Number(s): NP_001020366.1; NP_001020365.1; NP_001257.4
Human GeneID(s): 1066
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).

Immunogen

Peptide with sequence C-NTQAAQKLKDKE, from the internal region (near C terminus) of the protein sequence according to NP_001020366.1; NP_001020365.1; NP_001257.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 60kDa band observed in lysates of cell line THP-1, approx. 55kDa in Human Duodenum lysates, and approx. 55-60kDa in Human Liver lysates (calculated MW of 62.6kDa according to NP_001020366.1). These molecular weights are observed by other commercial sources. Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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