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Goat Anti-CFC1 / Cripto-1 Antibody (EB06894)

Related Products: EBP06894 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06894 Goat Anti-CFC1 / Cripto-1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06894 1
EB06894 (2µg/ml) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897
Official Symbol: CFC1
Accession Number(s): NP_115934.1
Human GeneID(s): 55997

Immunogen

Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat

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