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Goat Anti-Chat (mouse) Antibody - Trial (EB07255-T)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07255-T | Goat Anti-Chat (mouse) Antibody - Trial | Pep-ELISA, WB |  |
20µg specific antibody in 40µl | Mouse | $105.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB07255 optimised QC. Primary incubation 1 hour at room temperature.
Images A and B: Mouse Brain and Testes lysate at primary Ab concentration 0.1ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.Target Protein
Principal Names: Chat, choline acetyltransferase, B230380D24RikOfficial Symbol: Chat
Accession Number(s): NP_034021.1
Non-Human GeneID(s): 12647 (mouse), 432359 (rat)
Important Comments: This antibody is NOT expected to cross-react to Human CHAT.
Immunogen
Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 75kDa band observed in Mouse Brain and Testis lysates, and approx. 70kDa in preliminary testing of Rat Testes and Mouse small intestine lysate (calculated MW of 72.3kDa according to Mouse NP_034021.1). Recommended concentration:0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Species Reactivity
Tested: MouseExpected from sequence similarity: Mouse, Rat