Change country
Home

Goat Anti-CHD7 Antibody (EB07994)

Related Products: EBP07994 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07994 Goat Anti-CHD7 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07994 1
EB07994 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3
Official Symbol: CHD7
Accession Number(s): NP_060250.2
Human GeneID(s): 55636
Non-Human GeneID(s): 320790 (mouse), 312974 (rat)

Immunogen

Peptide with sequence C-KDEIDEFANSPSE, from the internal region of the protein sequence according to NP_060250.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336kDa according to Human NP_060250.2 and 175kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Product Reviews

Please login to review this product.