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Goat Anti-CHD7 Antibody (EB07994)

Related Products: EBP07994 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07994 Goat Anti-CHD7 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
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EB07994 1
EB07994 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3
Official Symbol: CHD7
Accession Number(s): NP_060250.2
Human GeneID(s): 55636
Non-Human GeneID(s): 320790 (mouse), 312974 (rat)

Immunogen

Peptide with sequence C-KDEIDEFANSPSE, from the internal region of the protein sequence according to NP_060250.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336kDa according to Human NP_060250.2 and 175kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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