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Code Name Applications Availability Size Tested species Price Grade  
EB10236 Goat Anti-CIRBP (aa 161-172) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10236 1
EB10236 (0.01µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10236 2
EB10236 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CIRP, cold inducible RNA binding protein, cold inducible RNA-binding protein, Cold-inducible RNA-binding protein, glycine-rich RNA binding protein, CIRBP, A18 hnRNP
Official Symbol: CIRBP
Accession Number(s): NP_001271.1
Human GeneID(s): 1153
Non-Human GeneID(s): 12696 (mouse), 81825 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_001271.1) only.

Immunogen

Peptide with sequence C-RDSYDSYATHNE, from the C Terminus of the protein sequence according to NP_001271.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 18kDa band observed in Mouse Testis lysates (calculated MW of 18.6kDa according to Human NP_001271.1 and Mouse NP_031731.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Recommended concentration: 3-5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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