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Goat Anti-CKB / Brain Creatine Kinase Antibody (EB07185)

Related Products: EBP07185 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07185 Goat Anti-CKB / Brain Creatine Kinase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07185 1
EB07185 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CKB, brain creatine kinase, creatine kinase, brain, B-CK, CKBB, creatine kinase B-chain, creatine kinase-B
Official Symbol: CKB
Accession Number(s): NP_001814.2
Human GeneID(s): 1152
Non-Human GeneID(s): 12709 (mouse)

Immunogen

Peptide with sequence C-KPSDEHKTDLNPDN, from the internal region of the protein sequence according to NP_001814.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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