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Goat Anti-CKB / Brain Creatine Kinase Antibody (EB07185)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07185 | Goat Anti-CKB / Brain Creatine Kinase Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
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EB07185 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: CKB, brain creatine kinase, creatine kinase, brain, B-CK, CKBB, creatine kinase B-chain, creatine kinase-BOfficial Symbol: CKB
Accession Number(s): NP_001814.2
Human GeneID(s): 1152
Non-Human GeneID(s): 12709 (mouse)
Immunogen
Peptide with sequence C-KPSDEHKTDLNPDN, from the internal region of the protein sequence according to NP_001814.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:2000.Western blot: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow