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Goat Anti-CLIM1 / PDLIM1 Antibody (EB06229)

Related Products: EBP06229 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06229 Goat Anti-CLIM1 / PDLIM1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06229 1
EB06229 staining (0.1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06229 2
EB06229 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDLIM1, CLIM1, CLP36, ELFIN, CLP-36, hCLIM1, PDZ and LIM domain 1 (elfin), carboxy terminal LIM domain protein 1, PDZ and LIM domain 1, elfin, enigma
Official Symbol: PDLIM1
Accession Number(s): NP_066272.1
Human GeneID(s): 9124

Immunogen

Peptide with sequence C-TPPEGYEVVTVFPK, from the C Terminus of the protein sequence according to NP_066272.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in Human Lung lysate (calculated MW of 36.1kDa according to NP_066272.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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