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Goat Anti-CLLD8 / SETDB2 Antibody (EB05724)

Code Name Applications Availability Size Tested species Price Grade  
EB05724 Goat Anti-CLLD8 / SETDB2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB05724 1
EB05724 (0.3µg/ml) staining of Nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SETDB2, CLLD8, SET domain, bifurcated 2, CLLL8 protein, C13orf4, CLLL8, DKFZp586I0123, DKFZp761J1217, KMT1F, chronic lymphocytic leukemia deletion region 8, lysine N-methyltransferase 1F, histone-lysine N-methyltransferase SETDB2
Official Symbol: SETDB2
Accession Number(s): NP_114121.2; NP_001153780.1
Human GeneID(s): 83852
Important Comments: This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).

Immunogen

Peptide with sequence GEKNGDAKTFWME-C, from the N Terminus of the protein sequence according to NP_114121.2; NP_001153780.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 85kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9kDa according to NP_114121.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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