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Goat Anti-Clusterin / ApoJ (mouse) Antibody (EB08920)

Code Name Applications Availability Size Tested species Price Grade  
EB08920 Goat Anti-Clusterin / ApoJ (mouse) Antibody Pep-ELISA, IF, WB Yes 100µg specific antibody in 200µl Human, Rat $333.00 Elite

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EB08920 1
EB08920 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08920 2
EB08920 (0.3µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08920 3
EB08920 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message
Official Symbol: Clu
Accession Number(s): NP_038520.1
Non-Human GeneID(s): 12759 (mouse), 24854 (rat)


Peptide with sequence C-EKALQEYRRKSRAE, from the C Terminus of the protein sequence according to NP_038520.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 38kDa band observed in Rat Brain and Human Testis lysates.(calculated MW of 51.4kDa according to Rat XP_006252156.1) This molecular weight is routinely observed by other sources e.g. PMID: 11560857, Miyata et al. The 38kDa band is thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa in Rat Brain. This band was successfully blocked by incubation with the immunizing peptide.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Mouse, Rat

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