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Goat Anti-Cofilin 2 (muscle) Antibody - Trial (EB06889-T)

Related Products: EBP06889 - Immunizing Peptide, Goat Anti-Cofilin 2 (muscle) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06889-T Goat Anti-Cofilin 2 (muscle) Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Rat $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06889-T 1
EB06889 (0.003µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06889-T 2
EB06889 (0.001µg/ml) staining of Rat Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06889-T 3
HEK293 overexpressing Human Muscle Cofilin 2 (RC222215) and probed with EB06889 (mock transfection in first lane), tested by Origene.
EB06889-T 4
EB06889 (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: cofilin 2 (muscle), HGNC:1875, CFL2, NEM7, cofilin 2
Official Symbol: CFL2
Accession Number(s): NP_068733.1; NP_619579.1
Human GeneID(s): 1073
Important Comments: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)

Immunogen

Peptide with sequence C-DIKDRSTLGEK, from the C Terminus of the protein sequence according to NP_068733.1; NP_619579.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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