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Code Name Applications Availability Size Tested species Price Grade  
EB07914 Goat Anti-COX4I1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07914 1
EB07914 (0.01µg/ml) staining of human muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07914 2
EB07914 (2.5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07914 3
EB07914 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: COX4I1, cytochrome c oxidase subunit IV isoform 1, COX4, COXIV, MGC72016, COX4-1, COXIV-1, cytochrome c oxidase polypeptide IV
Official Symbol: COX4I1
Accession Number(s): NP_001852.1; NP_001305726.1
Human GeneID(s): 1327
Important Comments: This antibody is expected to recognize reported isoforms 1 and 4 (NP_001852.1; NP_001305726.1). Reported variants represent identical protein: NP_001305715.1, NP_001852.1. This product may cross-react to COX4I2 (GeneID 84701).

Immunogen

Peptide with sequence C-QGLASKWDYEKNE, from the C Terminus of the protein sequence according to NP_001852.1; NP_001305726.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 18kDa band observed in human skeletal muscle lysates (calculated MW of 19.6kDa according to NP_001852.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Thyroid and Testis. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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