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Goat Anti-CPT1A Antibody (EB07419)

Related Products: EBP05481 - Immunizing Peptide, EBP07419 - Immunizing Peptide, Goat Anti-CPT1A Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07419 Goat Anti-CPT1A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07419 1
EB07419 (2µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07419 2
EB07419 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07419 3
EB07419 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CPT1A, carnitine palmitoyltransferase 1A (liver), CPT1, CPT1-L, L-CPT1, carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase I, carnitine palmitoyltransferase I, liver
Official Symbol: CPT1A
Accession Number(s): NP_001867.2; NP_001027017.1
Human GeneID(s): 1374
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.

Immunogen

Peptide with sequence C-DPAQTVEQRLKLFK, from the internal region of the protein sequence according to NP_001867.2; NP_001027017.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80-85kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2kDa according to NP_001027017.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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