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Goat Anti-CPT2 (aa141-154) Antibody (EB09736)

Related Products: EBP09736 - Immunizing Peptide, EBP09737 - Immunizing Peptide, Goat Anti-CPT2 (aa406-418) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09736 Goat Anti-CPT2 (aa141-154) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09736 1
EB09736 (2µg/ml) staining of Human Liver (A), Mouse Liver (B) and (1ug/ml) on Rat Heart (C) lysate (35µg protein in RIPA buffer). Detected by Chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CPT2, carnitine palmitoyltransferase II, CPT1, CPTASE, OTTHUMP00000010493, carnitine O-palmitoyltransferase
Official Symbol: CPT2
Accession Number(s): NP_000089.1
Human GeneID(s): 1376

Immunogen

Peptide with sequence C-PKSEYNDQLTRATN, from the internal region of the protein sequence according to NP_000089.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Rat Heart and in Mouse Liver lysates and approx 70-75kDa doublet in Human Liver lysates (calculated MW of 73.8kDa according to Human NP_000089.1 and 74.0kDa according to Mouse NP_034079.2 and Rat NP_037062.1) An additional band of unknown identity was consistently observed at 25-27kDa in some Mouse and in Human Liver lysates and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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