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Goat Anti-creatine kinase M-type (aa258-270) Antibody (EB11997)

Related Products: EBP11996 - Immunizing Peptide, EBP11997 - Immunizing Peptide, Goat Anti-creatine kinase M-type Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11997 Goat Anti-creatine kinase M-type (aa258-270) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
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EB11997 1
EB11997 (0.3µg/ml) staining of Human (A) Pig B) and (0.1ug/ml) Rat (C) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CKM, creatine kinase, muscle, CKMM, M-CK, creatine kinase M chain, creatine kinase M-type, creatine kinase-M
Official Symbol: CKM
Accession Number(s): NP_001815.2
Human GeneID(s): 1158
Non-Human GeneID(s): 12715 (mouse), 24265 (rat)

Immunogen

Peptide with sequence C-QKIEEIFKKAGHP, from the internal region of the protein sequence according to NP_001815.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 45kDa band observed in Human and Pig Heart lysates, and approx 40kDa in Rat Heart lysates (calculated MW of 43.1kDa according to Human NP_001815.2 and 43kDa according to Rat NP_036662.1 and Pig NP_001123421.1). Recommended concentration:0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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