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Goat Anti-creatine kinase M-type Antibody (EB11996)

Related Products: EBP11996 - Immunizing Peptide, EBP11997 - Immunizing Peptide, Goat Anti-creatine kinase M-type (aa258-270) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11996 Goat Anti-creatine kinase M-type Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11996 1
EB11996 (0.01µg/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11996 2
EB11996 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CKM, creatine kinase, muscle, CKMM, M-CK, creatine kinase M chain, creatine kinase M-type, creatine kinase-M
Official Symbol: CKM
Accession Number(s): NP_001815.2
Human GeneID(s): 1158

Immunogen

Peptide with sequence NTHNKFKLNYKPEE-C, from the N Terminus of the protein sequence according to NP_001815.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

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