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Goat Anti-CREB1 (aa96-109) Antibody (EB12171)

Related Products: EBP12171 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12171 Goat Anti-CREB1 (aa96-109) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12171 1
EB12171 (0.1µg/ml) staining of Jurkat (A) and NIH3T3 (B) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12171 2
EB12171 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: CREB1, cAMP responsive element binding protein 1, CREB, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein
Official Symbol: CREB1
Accession Number(s): NP_004370.1; NP_604391.1
Human GeneID(s): 1385
Non-Human GeneID(s): 12912 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004370.1; NP_604391.1).

Immunogen

Peptide with sequence C-DSQESVDSVTDSQK, from the internal region of the protein sequence according to NP_004370.1; NP_604391.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 2.5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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