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Goat Anti-CRKL Antibody (EB05254)

Code Name Applications Availability Size Tested species Price Grade  
EB05254 Goat Anti-CRKL Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

Ordering Information for

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$49.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05254 1
EB05254 (0.01µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05254 2
EB05254 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like, OTTHUMP00000198539
Official Symbol: CRKL
Accession Number(s): NP_005198.1
Human GeneID(s): 1399

Immunogen

Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in lysates of cell lines K562 (calculated MW of 33.8kDa according to NP_005198.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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