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Goat Anti-CSF1R (aa 700-710) Antibody (EB10257)

Related Products: EBP10257 - Immunizing Peptide, EBP10258 - Immunizing Peptide, Goat Anti-CSF1R (aa734-744) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10257 Goat Anti-CSF1R (aa 700-710) Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10257 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with EB10257 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10257 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD115, CD115 antigen, C-FMS, colony stimulating factor 1 receptor, CSFR, FIM2, FMS, FMS proto-oncogene, macrophage colony stimulating factor I receptor, McDonough feline sarcoma viral (v-fms) oncogene homolog, CSF1R
Official Symbol: CSF1R
Accession Number(s): NP_005202.2
Human GeneID(s): 1436
Non-Human GeneID(s): 12978 (mouse), 307403 (rat)

Immunogen

Peptide with sequence C-KNIHLEKKYVR, from the internal region of the protein sequence according to NP_005202.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing full-length Human CSF1R (myc and DYKDDDDK tagged), a band of approx. 200kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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