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Goat Anti-CSX1 / NKX2-5 Antibody (EB09412)

Code Name Applications Availability Size Tested species Price Grade  
EB09412 Goat Anti-CSX1 / NKX2-5 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $324.00 Elite

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EB09412 1
EB09412 (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09412 2
EB09412 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09412 3
EB09412 (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: NKX2-5, NK2 transcription factor related, locus 5 (Drosophila), CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1, NK2 transcription factor homolog E, NK2 transcription factor related, locus 5, cardiac-specific homeo box, tinman paralog
Official Symbol: NKX2-5
Accession Number(s): NP_004378.1; NP_001159647.1; NP_001159648.1
Human GeneID(s): 1482
Non-Human GeneID(s): 18091 (mouse), 114109 (rat)
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).


Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog

Specific Reference

This antibody has been successfully used in Western blot on Rat:
Sergio Li Calzi, Todd Cook, Domenico G. Della Rocca, Juan Zhang, Vinayak Shenoy, Yuanqing Yan, Andrew Espejo, Anandharajan Rathinasabapathy, Max H. Jacobsen, Tatiana Salazar, George E. Sandusky, Lynn C. Shaw, Keith March, Mohan K. Raizada, Carl J. Pepine, Michael J. Katovich, and Maria B. Grant
Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model
Stem Cells International Volume 2019, Article ID 3945850, 11 pages (2019)

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