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Goat Anti-Cyb5r4 (mouse aa444-458) Antibody (EB11476)

Related Products: EBP11476 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11476 Goat Anti-Cyb5r4 (mouse aa444-458) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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ARP American Research Products, Inc
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EB11476 1
EB11476 (0.1µg/ml) staining of Rat Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: 2810034J18Rik, b5/b5r, B5+B5R, b5b5r, C79736, cb5/cb5R, Cb5cb5r, Cyb5r4, cytochrome b5 reductase 4, cytochrome b-type NAD(P)H oxidoreductase, flavohemoprotein b5/b5R, NADPH cytochrome B5 oxidoreductase, Ncb5or, N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein
Official Symbol: Cyb5r4
Accession Number(s): NP_077157.2
Non-Human GeneID(s): 266690 (mouse)

Immunogen

Peptide with sequence RCQLEKLALREKRFD, from the internal region of the protein sequence according to NP_077157.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Mouse, Rat

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