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Goat Anti-Cytochrome b reductase 1 Antibody (EB06633)

Related Products: EBP06633 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06633 Goat Anti-Cytochrome b reductase 1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06633 1
EB06633 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06633 2
EB06633 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3
Official Symbol: CYBRD1
Accession Number(s): NP_079119.3
Human GeneID(s): 79901
Important Comments: This antibody is expected to recognise isoform 1 (NP_079119.3) only.

Immunogen

Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567.
IHC: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific References

This antibody has been successfully used in WB on Human:
Dostalikova-Cimburova M, Balusikova K, Kratka K, Chmelikova J, Hejda V, Hnanicek J, Neubauerova J, Vranova J, Kovar J, Horak J.
Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease.
J Cell Mol Med. 2014 Jun 3.
PMID: 24894955

This antibody has been successfully used in WB on Human:
Dostalikova-Cimburova M, Kratka K, Balusikova K, Chmelikova J, Hejda V, Hnanicek J, Neubauerova J, Vranova J, Kovar J, Horak J.
Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency.
J Cell Mol Med. 2012 Aug;16(8):1816-26.
PMID: 21973163

This antibody has been successfully used in WB on Human:
Balusikova K, Neubauerova J, Dostalikova-Cimburova M, Horak J, Kovar J.
Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess.
Mol Cell Biochem. 2009 Jan;321(1-2):123-33.
PMID: 18830567