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Code Name Applications Availability Size Tested species Price Grade  
EB11746 Goat Anti-cytokeratin 20 (aa48-60) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11746 1
EB11746 (0.03µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11746 2
EB11746 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CK20, CK-20, cytokeratin 20, cytokeratin-20, K20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT20, KRT21, MGC35423, OTTHUMP00000164518, protein IT
Official Symbol: KRT20
Accession Number(s): NP_061883.1
Human GeneID(s): 54474

Immunogen

Peptide with sequence C-NSRHTVNYGSDLT, from the internal region of the protein sequence according to NP_061883.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Colon. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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