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Goat Anti-D-amino-acid oxidase (aa286-298) Antibody (EB11100)

Code Name Applications Availability Size Tested species Price Grade  
EB11100 Goat Anti-D-amino-acid oxidase (aa286-298) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11100 1
EB11100 (0.03µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DAAO, D-amino-acid oxidase, DAMOX, MGC35381, OXDA, DAO
Official Symbol: DAO
Accession Number(s): NP_001908.3
Human GeneID(s): 1610

Immunogen

Peptide with sequence C-RPQIRLEREQLRT, from the internal region of the protein sequence according to NP_001908.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 39.5kDa according to NP_001908.3). Recommended concentration: 0.03-0.01µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific References

This antibody has been successfully used in WB on Macaques:
Nuzzo T, Punzo D, Devoto P, Rosini E, Paciotti S, Sacchi S, Li Q, Thiolat ML, Véga C, Carella M, Carta M, Gardoni F, Calabresi P, Pollegioni L, Bezard E, Parnetti L, Errico F, Usiello A
The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of Mptplesioned macaques and in the cerebrospinal fluid of Parkinson’s disease patients
Sci Rep. 2019 Jun 20;9(1):8898. 
PMID: 31222058

This antibody has been successfully used in WB and IF on Mouse:
Sasabe J, Miyoshi Y, Rakoff-Nahoum S, Zhang T, Mita M, Davis BM, Hamase K, Waldor MK
Interplay between microbial D-amino acids and host D-amino acid oxidase modifies murine mucosal defence and gut microbiota
Nat Microbiol. 2016 Jul 25;1(10):16125
PMID: 27670111

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