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Goat Anti-DBF4B (aa193-207) Antibody (EB10886)

Code Name Applications Availability Size Tested species Price Grade  
EB10886 Goat Anti-DBF4B (aa193-207) Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $255.00 Aspiring

Ordering Information for United States of America (change)

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Target Protein

Principal Names: DBF4B, DBF4 homolog B (S. cerevisiae), ASKL1, DRF1, FLJ13087, MGC15009, ZDBF1B, chifb, ASK-like protein 1, Dbf4-related factor 1, activator of S phase kinase-like protein 1, activator of S-phase kinase-like protein 1, chiffon homolog B, protein DBF4 homolog B, zinc finger, DBF-type containing 1B
Official Symbol: DBF4B
Accession Number(s): NP_663696.1; NP_079380.1
Human GeneID(s): 80174
Important Comments: This antibody is expected to recognize both reported isoforms.

Immunogen

Peptide with sequence QLSLASLCVKKQQPK, from the internal region of the protein sequence according to NP_663696.1; NP_079380.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments in Human Testis lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Species Reactivity

Tested:
Expected from sequence similarity: Human