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Code Name Applications Availability Size Tested species Price Grade  
EB09917 Goat Anti-DCUN1D1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09917 1
EB09917 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09917 2
EB09917 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae), DCUN1L1, RP42, RP42 homolog, SCCRO, SCRO, squamous cell carcinoma-related oncogene, Tes3, DCUN1D1
Official Symbol: DCUN1D1
Accession Number(s): NP_065691.2
Human GeneID(s): 54165
Non-Human GeneID(s): 114893 (mouse)

Immunogen

Peptide with sequence C-RPQIAGTKSTT, from the C Terminus of the protein sequence according to NP_065691.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 28kDa band observed in HepG2 lysates (calculated MW of 30.1kDa according to NP_065691.2). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

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