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Code Name Applications Availability Size Tested species Price Grade  
EB09922 Goat Anti-DDA1 Antibody ELISA Allow up to 10 days for delivery 100µg specific antibody in 200µl $255.00 Aspiring

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: C19orf58, cross-immune reaction antigen PCIA1, DET1 and DDB1 associated 1, MGC2594, PCIA1, DDA1
Official Symbol: DDA1
Accession Number(s): NP_076955.1
Human GeneID(s): 79016
Non-Human GeneID(s): 66498 (mouse)

Immunogen

Peptide with sequence C-PTREYPSEQIIVTE, from the internal region of the protein sequence according to NP_076955.1.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 25kDa band in Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.8kDa according to NP_076955.1. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Dog

Background Reference

Olma MH, Roy M, Le Bihan T, Sumara I, Maerki S, Larsen B, Quadroni M, Peter M, Tyers M, Pintard L,
An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases.
Journal of cell science 2009 Apr 122 (Pt 7): 1035-44.
PMID: 19295130

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