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Goat Anti-DDX5 / p68 RNA helicase Antibody - Trial (EB06310-T)

Related Products: EBP06310 - Immunizing Peptide, Goat Anti-DDX5 / p68 RNA helicase Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06310-T Goat Anti-DDX5 / p68 RNA helicase Antibody - Trial Pep-ELISA, WB Allow up to 10 days for delivery 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06310-T 1
EB06310 (0.5µg/ml) staining of cell lines A431(A), HeLa(B), HepG2(C), Jurkat(D) and NIH3T3 (E) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DKFZp434E109, DKFZp686J01190, ATP-dependent RNA helicase DDX5, DEAD box-5
Official Symbol: DDX5
Accession Number(s): NP_004387.1
Human GeneID(s): 1655
Non-Human GeneID(s): 13207 (mouse)

Immunogen

Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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