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Goat Anti-DEAD-box protein 6 Antibody (EB07126)

Related Products: EBP07126 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07126 Goat Anti-DEAD-box protein 6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07126 1
EB07126 (0.3µg/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07126 2
EB07126 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07126 3
EB07126 (5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
Official Symbol: DDX6
Accession Number(s): NP_004388.1
Human GeneID(s): 1656

Immunogen

Peptide with sequence C-AEYHSEPVEDEKP, from the C Terminus of the protein sequence according to NP_004388.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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