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Goat Anti-POLRMT Antibody (EB12137)

Related Products: EBP12137 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12137 Goat Anti-POLRMT Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
Click here to download PDF datasheet.

Target Protein

Principal Names: POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial
Official Symbol: POLRMT
Accession Number(s): NP_005026.3
Human GeneID(s): 5442

Immunogen

Peptide with sequence C-KETLQAVPKPGAFD, from the C Terminus of the protein sequence according to NP_005026.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave bands at approx 90kDa and 35kDa in Human HepG2 lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 139kDa according to NP_005026.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Species Reactivity

Tested:
Expected from sequence similarity: Human

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