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Goat Anti-DPM3 Antibody (EB08946)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB08946 | Goat Anti-DPM3 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
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EB08946 (2µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: DPM3, dolichyl-phosphate mannosyltransferase polypeptide 3, MGC125904, MGC125905, MGC34275, dolichol-phosphate mannose synthase subunit 3, dolichyl-phosphate beta-D-mannosyltransferase subunit 3, mannose-P-dolichol synthase subunit 3, prostin 1Official Symbol: DPM3
Accession Number(s): NP_061846.2; NP_714963.1
Human GeneID(s): 54344
Important Comments: This antibody is expected to recognize both reported isoforms (NP_061846.2 and NP_714963.1).
Immunogen
Peptide with sequence HDCEDAARELQSQ, from the internal region of the protein sequence according to NP_061846.2; NP_714963.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:1000.Western blot: Approx 10+13kDa bands observed in lysates of cell line K562 (calculated MW of 13.3kDa according to NP_061846.2 and 10.1kDa according to NP_714963.1). Recommended concentration: 1-3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig