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Goat Anti-DPM3 Antibody (EB08946)

Related Products: EBP05273 - Immunizing Peptide, EBP08946 - Immunizing Peptide, Goat Anti-DPM3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08946 Goat Anti-DPM3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB08946 1
EB08946 (2µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DPM3, dolichyl-phosphate mannosyltransferase polypeptide 3, MGC125904, MGC125905, MGC34275, dolichol-phosphate mannose synthase subunit 3, dolichyl-phosphate beta-D-mannosyltransferase subunit 3, mannose-P-dolichol synthase subunit 3, prostin 1
Official Symbol: DPM3
Accession Number(s): NP_061846.2; NP_714963.1
Human GeneID(s): 54344
Important Comments: This antibody is expected to recognize both reported isoforms (NP_061846.2 and NP_714963.1).

Immunogen

Peptide with sequence HDCEDAARELQSQ, from the internal region of the protein sequence according to NP_061846.2; NP_714963.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 10+13kDa bands observed in lysates of cell line K562 (calculated MW of 13.3kDa according to NP_061846.2 and 10.1kDa according to NP_714963.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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