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Goat Anti-DPP4 / CD26 Antibody - Trial (EB07835-T)

Code Name Applications Availability Size Tested species Price Grade  
EB07835-T Goat Anti-DPP4 / CD26 Antibody - Trial Pep-ELISA, WB, IHC, FC Yes 20µg specific antibody in 40µl Human, Rat $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

TestShot
EB07835-T 1
EB07835 (0.3µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07835-T 2
EB07835 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07835-T 3
EB07835 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
EB07835-T 4
EB07835 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DPP4, CD26, dipeptidyl-peptidase 4 (CD26, adenosine deaminase complexing protein 2), ADABP, ADCP2, DPPIV, TP103, T-cell activation antigen CD26, adenosine deaminase complexing protein 2, dipeptidylpeptidase 4 (CD26, adenosine deaminase complexing protein 2), dipeptidylpeptidase IV, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
Official Symbol: DPP4
Accession Number(s): NP_001926.2
Human GeneID(s): 1803
Non-Human GeneID(s): 13482 (mouse), 25253 (rat)

Immunogen

Peptide with sequence C-PPHFDKSKKYP, from the internal region of the protein sequence according to NP_001926.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 110kDa band observed in Rat Lung lysates (calculated MW of 88.3Da according to NP_001926.2). The observed molecular weight of this glycoprotein corresponds to earlier findings in literature with different antibodies (Herrera et al, J Biol Chem. 2001 Jun 1;276(22):19532-9.; PMID: 11278278). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 8µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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