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Goat Anti-DSS1 / SHFM1 Antibody (EB06156)

Related Products: EBP06156 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06156 Goat Anti-DSS1 / SHFM1 Antibody Pep-ELISA, ICC Yes 100µg specific antibody in 200µl $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Official Symbol: SEM1
Accession Number(s): NP_006295.1
Human GeneID(s): 7979
Non-Human GeneID(s): 20422 (mouse)

Immunogen

Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1µg/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8KDa according to NP_006295. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig

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