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Code Name Applications Availability Size Tested species Price Grade  
EB06940 Goat Anti-Duffy / FY / DARC Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB06940 1
EB06940 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06940 2
EB06940 (5µg/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.
EB06940 3
EB06940 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB06940 4
Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Target Protein

Principal Names: ACKR1, atypical chemokine receptor 1 (Duffy blood group), CCBP1, CD234, DARC, Dfy, FY, GPD, GpFy, WBCQ1, Duffy antigen chemokine receptor, Duffy blood group antigen, Duffy blood group, atypical chemokine receptor, Duffy blood group, chemokine receptor, Fy glycoprotein, duffy blood group chemokine receptor, glycoprotein D, plasmodium vivax receptor, CTA-134P22.3, HGNC:4035
Official Symbol: ACKR1
Accession Number(s): NP_001116423.1; NP_002027.2
Human GeneID(s): 2532
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).

Immunogen

Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1; NP_002027.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3µg/ml. Paraffin embedded Human Lung. Recommended concentration: 5µg/ml.

Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human