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Goat Anti-DUSP1 Antibody (EB05197)

Code Name Applications Availability Size Tested species Price Grade  
EB05197 Goat Anti-DUSP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05197 1
EB05197 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05197 2
EB05197 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05197 3
EB05197 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DUSP1, dual specificity phosphatase 1, HVH1, CL100, MKP-1, PTPN10, serine/threonine specific protein phosphatase, MKP1
Official Symbol: DUSP1
Accession Number(s): NP_004408.1
Human GeneID(s): 1843
Non-Human GeneID(s): 19252 (mouse)

Immunogen

Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40kDa band observed in lysates of HeLa (calculated MW of 39.3kDa according to NP_004408.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow, Mouse, Rat

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